Rich Shukle

Assistant Professor (Adjunct) USDA-ARS Research Entomologist, Hessian Fly Genetics
 
Office Phone: 494-6351
Office Number: WSLR 220
Lab Number: WSLR 241
Office Phone: 494-7193
Email Address: shukle@purdue.edu


Education

PhDEntomology 1980, University of California, Davis


Research Interests

Program SummaryThe Hessian fly is a major pest of wheat in many of the production areas of the United States and other parts of the world. The use of resistant wheat is the most economically and environmentally sound method of control for this pest. However, the use of resistant cultivars has resulted in the selection of biotypes that can survive on formally resistant wheat. An understanding of the molecular basis of biotype evolution will assist maintaining effective resistance in wheat. Research objectives directed toward understanding Hessian fly genomics include: 1) develop genetic transformation to enhance molecular analysis through transposon tagging, enhancer trapping, and gene validation; 2) use mitochondrial DNA sequence divergence to reveal historical and biogeographical events and population structure; 3) evaluate divergence in nuclear sequences among different geographic populations; 4) clone and characterize genes to determine cytological location, genomic organization, exon/intron structure, codon usage; 5) identify transgenes to enhance resistance in wheat.


Professional Activities

1994 Participated in the session on genetic diversity in insects at the IPRI Workshop, Stillwater, OK.

2002 Organized a session on functional genomics in insect/plant interactions for the 2002 IPRI Workshop.


Extension/Outreach Activities

Currently developing a collaborative activity with individuals in Syria/Spain to investigate geographic distribution of mtDNA haplotypes, virulence, and population structure of Hessian fly from West Asia, North Africa, and southern Europe.


Selected Publications

Refereed:
Shukle, R. H. and Stuart, J. J. Physical mapping of DNA sequences in the Hessian fly, Mayetiola destructor. J. Heredity 86:1-5, 1995.
Shukle, R. H. and Russell, V. W. Mariner transposase-like sequences from the Hessian fly, Mayetiola destructor. J. Heredity 86:364-368, 1995.
Russell, V. W. and Shukle, R. H. Molecular and cytological analysis of mariner transposons from Hessian fly. J. Heredity 88:72-76, 1997.
Zantoko, L. and Shukle, R. H. Genetics of virulence in the Hessian fly to resistance gene H13 in wheat. J. Heredity 88:120-123, 1997.
Zantoko, L. and Shukle, R. H. An STS linked to a Hessian fly locus controlling virulence to resistance gene H13 in wheat. J. Heredity 90:242-247, 1999.
Shukle, R. H. Molecular and cytological characterization of an actin gene from Hessian fly (Diptera: Cecidomyiidae). Ann. Entomol. Soc. Am. 93:1166-1172, 2000.
Shukle, R. H. and Wu L. The role of protease inhibitors and parasitoids on the population dynamics of Sitotroga cerealella (Lepidoptera: Gelechiidae). Submitted to Environ. Entomol. 2001. Accepted with revision November 2001
Shukle, R. H. and Yoshiyama, M. Mitochondrial haplotypes and biogeography of Hessian fly (Diptera: Cecidomyiidae). Submitted Ann. Entomol. Soc. Am. 2002.
Yoshiyama, M. and Shukle, R. H. Cloning and characterization of a white gene from Hessian fly, Mayetiola destructor. Submitted Insect Mol. Biol. 2002.
Book Chapters/Proceedings:
Shukle, R. H. and Quiroz, C. Use of the electronic monitor for the study of aphids as vectors of barley yellow dwarf virus (BYDV). (Symposium Book Chapter) In: Proceedings: Thomas Say Publications in Entomology. History, Development and Application of AC Electronic Insect Feeding Monitors (Elisbury, M.M., Backus, E. A., and Ullman, D. L. eds.). Entomological Society of America, Lanham, Maryland; 106-121, 1994.