|
|
| ||||||
| |
| Professor Emeritus | |
|
Office Phone: 494-4612 Office Number: SMTH 135 Lab Number: SMTH 134 Office Phone: 494-4612 Email Address: rshade@purdue.edu |
| BS | Biology 1961, Ball State Univ |
| MS | Entomology 1966, Purdue Univ |
| Program Summary | Prior to 1985 the plant resistance to insect project used the traditional approach for developing crop plants with insect resistance. This phase of the project culminated with the release of three insect resistant germplasm lines: 1) a glandular-haired alfalfa germplasm with resistance to several insect pests of alfalfa (81IND-2), and 2 & 3) a maize inbred line and a maize Iowa stiff stalk synthetic population with resistance to the corn leaf aphid. Since 1996, 81IND-2 has been the major source of potato leafhopper resistance in all of the newest alfalfa varieties. Since 1985, the plant resistance to insect project has been focused on developing and managing cowpea weevil biological assays related to prospecting for genes whose products are insecticidal proteins (The project was part of a an experiment that transformed garden pea with the alpha-amylase inhibitor gene from red kidney bean, conferring immunity to the garden pea against the cowpea weevil and azuki bean weevil). Other major areas of interest are the development of strategies for gene deployment, and the development of a simple population dynamics model for predicting the impact of genes conferring cowpea weevil resistance. The population model is also used to identify stages of the cowpea weevil that are most vulnerable to plant resistance approaches. |
| 1994-1997 Demonstration of the Purdue Insect Feeding Monitor at Bug Bowl |
| 1997 Gamma Sigma Delta |
| Since (and prior to 1994), there has been collaboration with the B/C CRSP project for post harvest storage of cowpeas in Cameroon. Collaboration was related to screening cowpea varieties, land races and wild cowpea species for cowpea weevil resistance, as well as attempting to increase the level of resistance in one cowpea weevil resistant cowpea variety (TVu 2027). Bioassays were also conducted on advanced cowpea lines to reduce the risk of releasing new lines highly susceptible to the cowpea weevil. A new technique was developed for disinfesting cowpea seeds using solar energy. This technique is now commonly used in Cameroon and neighboring countries. |
| ENTM 630 | Plant Resistance to Insects |
| ENTM 691 | Special Prob. (Plant Resistance to Insects) |
| Refereed: |
| Zhu, K., J. E. Huesing, R. E. Shade and L. L. Murdock. 1994. Cowpea trypsin inhibitor and resistance to cowpea weevil (Coleoptera: Bruchidae) in cowpea variety "TVu 2027". Environ. Entomol. 23: 987-991. |
| Shade, R. E., H. E. Schroeder, J. J. Pueyo, L. M. Tabe, L. L. Murdock and M. J. Chrispeels. 1994. Transgenic pea seed expressing the alpha-amylase inhibitor of the common bean are resistant to bruchid beetles. Bio/Technology 12: 793-796. |
| Pittendrigh, B. R., J. E. Huesing, R. E. Shade, and L. L. Murdock. 1997. Effects of lectins, CRY1A/CRY1B Bt B-endotoxin, PAPA, protease and a-amylase inhibitors, on the development of the rice weevil, Sitophilus oryzae, using an artificial seed bioassay. Entomologia Experimentalis et Applicata. 82: 201-211. |
| Pittendrigh, B.R., J.E. Huesing, R.E. Shade, and L.L. Murdock. 1997. Supernumerary molts in the rice weevil, Sitophilus oryzae (L.), under low humidity Oecologia. 83: 225-231. |
| Zhu, K., J.E. Huesing, R.E. Shade, R. Bressan, P.M. Hasegawa and L.L. Murdock. 1996. An Insecticidal GlCNac - Specific Lectin Gene from Griffonia simplicifolia (Leguminoseae). Plant Physiol. 110: 195-202. |
| Shade, R.E., L.L. Murdock, and L.W. Kitch. 1996. The selection of a cowpea weevil biotype virulent to cowpea-weevil-resistant cowpea TVu 2027. J. Econ. Entomol. 89: 1325-1331. |
| Ntoukam, G., L.W. Kitch, R.E. Shade, and L.L.Murdock. (1997). A novel method for conserving cowpea germplasm and breeding stocks using solar disinfestation. J. Stored Prod. Res. 33: 175-179. |
| Kitch, L. W., and R. E. Shade. (1997) A novel bulk screening technique for detecting resistance to Callosobruchus maculatus in seeds of cowpea, Vigna unguiculata (submitted for publication). |
| Koiwa, H., R. E. Shade, K. Zhu-Salzman, L. Subramanian, S. S. Nielsen, L. L. Murdock, R. A. Bressan and P. M. Hasegawa. 1998. Phage display selection can differentiate insecticidal activity of soybean cystatins. (accepted for pub. in Proceedings of the National Academy of Science). |
| Shade, R. E., L. L. Murdock and L. W. Kitch. 1998. Interactions between cowpea weevil populations and Vigna species. (Submitted to J. Econ.Entomol, AES# 15773). |
| Zhu-Salzman, K., R. E. Shade, H. Koiwa, R. A. Salzman, M. Narasimhan, R. A. Bressan, P. M. Hasegawa and L. L. Murdock. 1998. Carbohydrate-binding and resistance to proteoloysis control insecticidal activity of Griffonia simplicifolia Lectin II (GS-II). Proceedings of the National Academy of Sciences (in press). |
| Shade, R. E., L. L. Murdock and L. W. Kitch. 1999. Interactions between cowpea weevil (Coleoptera: Bruchidae) populations and Vigna (Leguminosae) species. J. Econ. Entmol. 92(3): 740-745. |
| Koiwa, H., M.P. D'Urzo, K. Zhu-Salzman, J. I. Ibeas, R.E. Shade, L.L. Murdock, R.A. Bressan and P.M. Hasegawa. 2000. An In-gel assay of a recombinant western corn rootworm (Diabrotica virgivera virgifera) cysteine proteinase expressed in yeast. Anal Biochem. 282 (1): 153-155. |
| Koiwa, H., R.E. Shade, K. Zhu-Salzman, et al. 2000. A plant defensive cystatin (soyacystatin) targets cathepsin L-like digestive cysteine proteinases (DvCALs) in the larval midgut of western corn rootworm (Diabrotica virgifera virgifera). Febs. Lett. 471 (1): 67-70. |
| Koiwa H, D'Urzo MP, Assfalg-Machleidt I, Zhu-Salzman K, Shade RE, An HJ, Murdock LL, Machleidt W, Bressan RA, Hasegawa PM (2001) Phage display selection of hairpin loop soyacystatin variants that mediate high affinity inhibition of a cysteine proteinase. Plant J. 27, 393-391. |
| Murdock LL, and Shade RE (2002) Lectins and protease inhibitors as plant defenses against insects. Ag Food Chem (special issue, in press). |
| Book Chapters/Proceedings: |
| Murdock, L. L., R. E. Shade, L. W. Kitch, G. Ntoukam, J. Lowenberg-Deboer, J. E. Huesing, W. Moar, O. L. Chambliss, C. Endondo, and J. L. Wolfson. 1997. Postharvest storage of cowpea in sub-Saharan Africa. In B. B. Singh et al. [eds.] Advances in Cowpea Research. Copublication of IITA and JIRCAS. IITA, Ibadan, Nigeria. |
| Kononowicz, A. K., K. T. Cheah, M. L. Narasimhan, L. L. Murdock, R. E. Shade, M. J. Chrispeels, E. Filippone, L. M. Monti, R. A. Bressan, and P. M. Hasegawa. 1997. Developing a transformation system for cowpea (Vigna ungiculata [L.] Walp. In B. B. Singh et al. [eds.] Advances in Cowpea Research. Copublication of IITA and JIRCAS. IITA, Ibadan, Nigeria. |
| Patents (issued and/or pending): |
| Shade / Murdock / Furgason. Awarded U.S. Patent 4,809,554 for the "Purdue Ultrasonic Insect Detector". |
| Nielsen, S. / Hasagawa / Bressan / Murdock / Shade. 1994 (Patent Pending)"Soybean Cystein Proteinase Inhibitor". Inst. Ref. No. P93077. RCT has paid for an option to look at the potential of this gene. |
| Murdock / Kitch / Nielsen / Shade. 1999. (Patent Pending) "Sweet Cowpea: high sucrose grain" Inst. Ref. No. P99036. |
