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 | Professor, Insect Molecular Genetics | |
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Office Phone: 494-4561 Office Number: WSLR 132 Lab Number: WSLR 119 Office Phone: 494-1438 Email Address: stuartjj@purdue.edu |
| BA | Biology 1979, Washburn University |
| MS | Entomology 1982, Kansas State University |
| PhD | Entomology 1987, Kansas State University |
| Program Summary | My laboratory is focused on investigating insect genes that confer a selective advantage to pest species over insect pest management strategies and control methods. These investigations involve studies that both identify these important genes at the molecular level and elucidate their intergenic and environmental interactions in pest populations. These studies will have significant impact on the development of insect pest management strategies and promise to prolong the efficacy of important pest control measures. In the process, our studies will increase our knowledge of insect genomic organization. As experimental models, this work is focused on the Hessian fly, Mayetiola destructor, the coffee berry borer, Hypothenemus hampei, and the red flour beetle, Tribolium castaneum (Tribolium). Our primary focus is on the Hessian fly. This research is devoted to mapping and cloning avirulence genes that allow the pest to overcome the resistance conferred by specific resistance genes in wheat. Coffee berry borer research is focused on determining its population structure and developing molecular genetic markers that can be used in understanding the population biology of this insect. Tribolium research is dedicated to the improvement of its genetic map and the discovery of genes that underlie insecticide resistance in beetles. |
| 1994-2001 Reviewed for the following Journals: Annals of the ESA, Journal of Entomological Science, Environmental Entomology, The Great Lakes Entomologist., Journal of Pesticide Science, Bulletin of Entomological Research. 1994-2001 Ad hoc reviews USDA NRI Competitive Research Grants Program/ Plant Pest Interactions and Animal Health 1994-2002 Book review for the Entomological Society of America: Understanding and Controlling the German Cockroach, M.K. Rust, J.M. Owens and D.A. Reierson eds., Oxford University Press, 1995. 1994 USDA Plant Molecular Biologist Selection Committee 1996 NSF Young Scholars Program 1996 USDA Plant Molecular Biologist Selection Committee 1998 Marine Biotechnology, National Sea Grant College Program Grant Proposal Ad hoc Review. 1998-2000 Kansas State University Plant Biotechnology Center Proposal Ad hoc Review. 1999 Review for Biotechnology and Biological Sciences Research Council Grant. 1999 Review for South Carolina Agriculture and Forestry Research System proposed CSREES project. 1999 Ad hoc reviewer for two proposals for The Leverhulme Trust, 1 Pemberton Row, London EC4A 3BC. 2000 Ad hoc reviewer for a proposal for the International Centre for Genetic Engineering and Biotechnology. 2000 NIH panel Tropical Medicine and Parasitology study section 2001 Attended “Comparative Insect Genomics Workshop” Arlington, Virginia 2002 Reviewed paper for Genetics and proposal for Colciencias (Colombia) |
| 2000 Purdue Agriculture Team Award |
| Rice gall midge genetics and resistance management in India- I was contacted by Dr. Modan Mohan and Dr. Suresh Nair of the International Centre for Genetic Engineering and Biotechnology (ICGEB) in Delhi, and Dr. Sarat Sahu at the Rice Genome Laboratory, Central Rice Research Institute (CRRI) in Cuttack, India to develop a collaboration in the area of plant resistance to gall midge species- specifically the Hessian fly (Mayetiola destructor) and the Asian rice gall midge (Orseolia oryzae). Dr. Mohan visited my laboratory in 1998, and to strengthen this collaboration further, I received a Global Initiatives Grant to visit their laboratories in India in 1999. In 2001, Dr. Nair came to Purdue and we worked together in my laboratory to sequence 300 kb of DNA encompassing one of the rice gall midge resistance genes in rice (Gm2) that was cloned by Dr. Mohan and Dr. Nair. Dr. Sahu is currently developing a mapping population of the rice gall midge that will allow us to determine if virulence to Gm2 is the result of a gene-for-gene interaction and if synteny between Hessian fly and rice gall midge exists. Coffee berry borer genetics and resistance management- I was contacted by Dr. Luc Brun in 1996 to investigate the cytogenetics of the coffee berry borer, Hypothenemus hampei, (CBB), the most important insect pest of coffee in the world. We published the results of our collaborative investigations with Dr. Richard ffrench-Constant in Nature and PNAS. Through this notoriety, a Colombian scientist, Pablo Benavides, came to my laboratory as a graduate student to initiate a collaborate with Cenicafé. This collaboration now includes Dr. Fernando Vega of the USDA, Beltsville, MD. and we are investigating the genetics and population structure of this insect. Toward that goal, we have collected CBB samples from nearly every coffee producing country in the world and evaluated their genetic variability using AFLP-PCR as a DNA fingerprinting technique. Results so far suggest how this pest was introduced into Latin America and have provided genetic markers for future genetic and population genetic investigations. Other international activities include a trip to Brazil in 2000 to present a seminar and visit with potential collaborators at EMBRAPA in Sete Lagos and Uberlandia. I have had two students from Brazil, Giovani Mocelin and Fernando Valicente. Fernando is an emplyee of EMBRAPA in Sete Lagos. I attended a Spanish immersion school for 3 weeks in Costa Rica in 2001 and visited Oaxaca Mexico for two weeks in 2001 to improve my proficiency in Spanish. |
| ENTM 310 | Careers in Entomology |
| AGRY 310 | Genetics |
| BIOL 495 | Special Problems Undergrads in Biology |
| ENTM 498 | Special Problems in Entomology |
| ENTM 692A | Insect Genetics |
| ENTM 692 | Insect Ecology |
| ENTM 592 | Insect Physiology Laboratory |
| ENTM 692 | Laboratory in Insect Genetics |
| Refereed: |
| Shukle, R.H. and J.J. Stuart. 1995. Physical mapping of DNA sequences in the Hessian fly, Mayetiola destructor. J. Hered. 86:1-5. |
| Mocelin, G. and J.J. Stuart. 1995. Cytogenetics of chromosome rearrangements in the beetle Tribolium castaneum. Genome. 38:673-680. |
| Brun, L.O., J.J. Stuart, P. Borsa, V. Gaudichon, K. Aronsein and R.H. ffrench-Constant. 1995. Functional haplodiploidy. Nature. 374:506. |
| Brun, L.O., J.J. Stuart, V. Gaudichon, K. Aronsein, C. Coustau, P. Borsa and R.H. ffrench-Constant. 1995. Functional haplodiploidy: a novel mechanism for the spread of insecticide resistance in an important international insect pest. Proc. Nat. Acad. Sci. 92:9861-9865. |
| Mocelin, G. and J. J. Stuart. 1996. Synthesis and genetic manipulation of crossover suppressors in the beetle Tribolium castaneum. J. Hered. J. Hered. 87:27-34. |
| Stuart, J.J., M. De Gortari, P. S. Hall, M. E. Maxwell, G. Mocelin and W. M. Muir. 1996. Snapback, a midrepetitive element, produces DNA fingerprints in the beetle Tribolium castaneum. Genome. 39:568-578. |
| Formusoh, E.S., J.H. Hatchett, W.C. Black and J.J. Stuart. 1996. Sex-linked inheritance of virulence against wheat resistance gene H9 in the Hessian fly (Diptera: Cecidomyiidae). Ann. Entomol. Soc. Amer. 89:428-434. |
| Beeman, R.W., J.J. Stuart, S. Hass and K. Friesen. 1996. Chromosome extraction and revision of the second linkage group of Tribolium castaneum. J. Hered. 87:224-232. |
| Stuart, J.J., S. Welso and R. Ratcliffe. 1997. Chromosome rearrangements in the Hessian fly, Mayetiola destructor. Ann. Entomol. Soc. Am. 90:480-486. |
| Sammons, A. E., C. R. Edwards, L. W. Bledsoe, P. J. Boeve and J. J. Stuart. 1997. Behavioral and feeding assays reveal a western corn rootworm (Coleoptera: Chrysomelidae) variant that is attracted to soybean. J. Environ. Entomol. 26:1336-1342. |
| Stuart, J. J. S. J. Shulte, P. S. Hall, K. M. Mayer. 1998. Hessian fly avirulence gene mapping by bulked segregant analysis. Genome 41:702-708. |
| Stuart, J. J., S. Ray, B. J. Harrington, J. J. Neal, R. W. Beeman. 1998. Genetic mapping of a major locus controlling pyrethroid resistance in Tribolium castaneum. J. Econ. Entomol. 91:1232-1238. |
| Schulte, S. J., S. D. Rider, Jr., J. H. Hatchett and J. J. Stuart. 1999. Molecular genetic mapping of three X-linked avirulence genes, vH6, vH9 and vH13, in the Hessian fly. Genome 41:821-828. |
| Rider, S. D., Jr., W. Sun, R. H. Ratcliffe and J. J. Stuart. 2002. Chromosome landing near avirulence gene vH13 in the Hessian fly. Genome: Submitted. |
| Brown, S. J., J. P. Fellers, T. D. Shippy, E. Richardson, M. W. Maxwell, J. J. Stuart and R. E. Denell. 2002. Sequence Analysis of the Tribolium castaneum HOM-C: The region corresponding to the ANT-C. Genetics: Submitted. |
| Vega, F. E., P. Benavides, J. J. Stuart and S. L. O’Neill. 2002. Presence of Wolbachis in the coffee berry borer, Hypotheniumus hampei (Ferrari). Ann. Entomol. Soc. Am. Submitted. |
| Book chapters/Proceedings: |
| Cambron, S. & J. J. Stuart, Genetic evidence for the obviation of resistance in wheat by virulent Hessian fly biotypes, ESA Annual Meeting, Dallas, TX. Dec. 1994. |
| Rider, S.D, Jr. & J.J. Stuart, RE-banding and X chromosome polymorphism in the German cockroach, ESA Annual Meeting, Dallas, TX. Dec. 1994. |
| Mayer, K., P.H. Hall and J.J. Stuart, Chromosome location of Hessian fly virulence to resistance gene H6. Sigma Xi, Purdue University, 1996. |
| Roy, S., G. Mocelin, J.J. Neal and J.J. Stuart, Cosegregation of a P450 gene cluster and pyrethroid resistance in Tribolium. Sigma Xi, Purdue University, 1996. |
| Shulte, S., M.J. DeGortari and J.J. Stuart, Molecular analysis of a putative transposon in Tribolium. Sigma Xi, Purdue University, 1996. |
| Sammons, A.E., C.R. Edwards, J.J. Stuart, L.W. Bledsoe, P.J. Boeve, Behavioral and feeding assays reveal a western corn rootworm variant that is attracted to soybean. North Central Branch of the ESA, Columbus, OH. March 1997. |
| Schulte, S.J., P.S. Hall, K. Mayer and J.J. Stuart, Molecular genetic mapping of Hessian fly virulence to resistance genes in wheat. North Central Branch of the ESA, Columbus, OH. March 1997. |
| Sammons, A.E., C.R. Edwards, L.W. Bledsoe and J.J. Stuart, A western corn rootworm variant attracted to soybean. ESA Annual Meeting, Nashville, TN. Dec. 1997. |
| Schulte, S.J., P.S. Hall, K. Mayer and J.J. Stuart, Identification of a molecular marker linked to vH6, an X-linked avirulence gene in the Hessian fly. ESA Annual Meeting, Nashville, TN. Dec. 1997. |
| Sun, W., S. Ray, S.D. Rider and J.J. Stuart, A Cytochrome p450 gene cluster is associated with pyrethroid resistance in the beetle, Tribolium castaneum. International Symposium on Molecular Insect Science, June 1998. |
| Rider, S.D. Jr., S.J. Schulte, J.J. Stuart. Genetic and genomic mapping of avirulence genes vH6 and vH9 in the Hessian fly. International Symposium on Molecular Insect Science, June 1998. |
| Stuart, J.J. 1998. Looking for resistance mechanisms in "model" insects. Purdue Pesticides Programs, Spotlight on Purdue University. The Label, July 1998. |
| Stuart, J.J. 1999. Hessian fly avirulence genes. Annual Wheat Newsletter. |
| Collier, C., J. J. Stuart, S. Fasoula, T. Liang and C. Williams. 1999. Molecular aspects of Hessian fly resistance. Annual Wheat Newsletter. |
| Rider, S. D. Jr., W. Sun, F. H. Valicente, R.H. Ratcliffe and J. J. Stuart. 2000. An integrated physical and AFLP-based genetic map of the Hessian fly. Annual Wheat Newsletter. |
| Rider, S.D. Jr., W. Sun, F.H. Valicente and J.J. Stuart. 1999. Molecular genetic maping in the Hessian fly. ESA Annual Meeting, Atlanta, GA. Dec. 1999. |
| Rider, S.D. Jr., W. Sun, F.H. Valicente and J.J. Stuart. An AFLP based molecular genetic map of the Hessian fly. International Plant Resistance to Insects- 14th Biennial Workshop, March 2000. |
| Rider, S.D. Jr., W. Sun, F.H. Valicente, R.H. Ratcliffe and J.J. Stuart. Molecular genetic maping in the Hessian fly. International Congress of Entomology, Iguassu Falls, Brazil Aug. 2000. |
| Extension Publications: |
| Edwards, C.R., A.E. Sammons, L.W. Bledsoe, J.J. Stuart and P.J. Boeve. 1996. IPM responding to changes in pest dynamics: Western corn rootworm in first-year corn following soybean. In: North Central Region Extension Publication NCR 599, 1996 Integrated Pest Management in the North Central Region. |
| Stuart, J.J. 1998. Looking for resistance mechanisms in "model" insects. Purdue Pesticides Programs, Spotlight on Purdue University. The Label, July 1998. |
